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KMID : 0368420070500010012
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Journal of Plant Biology
2007 Volume.50 No. 1 p.12 ~ p.12
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Transcript profile of transgenicArabidopsis constitutively producing methyl jasmonate
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Jung Choon-Kyun
Yeu Song-Yion
Koo Yeon-Jong
Kim Min-Kyun
Choi Yang-Do
Cheong Jong-Joo
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Abstract
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Using an Affymetrix GeneChip™ containing 8300 oligonucleotide probes, we measured transcript levels in transgenicArabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than a two-fold increase or decrease) in the expression levels of 168 genes. Among them, 80 were up-regulated, including those involved in defense, oxidative stress-tolerance, and senescence. In contrast, the expression of 88 genes, including those that function in photosynthesis and cold/drought-stress responses, was significantly down-regulated. Thus, endogenous generation of methyl jasmonate through AtjMT-overexpression modified the transcript levels of genes previously identified as being jasmonate-responsive. This result confirms that MeJA formation is a key control point for jasmonate-responsive gene expression in plants.
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KEYWORD
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gene transcript profile, jasmonic acid methyltransferase, methyl jasmonate, microarray, transgenicArabidopsis
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